Some Biochemical Properties of Extracts Obtained from Rubia Tinctorum L. Leaves and Flowers
Keywords:
Rubia tinctorum, antioxidant, acetylcholine esterase, butyrylcholine esterase, extractAbstract
This study aimed to reveal the antioxidant properties of extracts obtained from the flowers and leaves of Rubia tinctorum L. (RT) and their effects on AChE and BChE enzyme activities. Furthermore, the content analysis of the ethanol extract was performed using LC/MS-MS. The methanol extract was found to have the highest total phenolic content with value of 105.23 ± 56.26 μg gallic acid /mg extract. FRAP analysis showed that the methanol extract had a higher reducing power, with a value of 2.52 μmol/mg extract, compared to other extracts. CUPRAC analysis results showed that the chloroform extract had the highest Cu(II) reducing capacity. The extract with the highest DPPH and ABTS radical scavenging activity was the methanol extract. The IC50 value determined for the methanol extract using the DPPH method was 26.47 μg/mL, and the trolox equivalent value determined using the ABTS method was 796 μg/mg extract. Enzyme inhibition results showed that the ethanol extract had the strongest activity for AChE and BChE, with IC50 values of 24.03 μg/mL and 40.89 μg/mL, respectively. The results demonstrate the antioxidant and enzyme inhibitory effects of RT extracts, suggesting that they may be beneficial in reducing oxidative stress and treating Alzheimer's disease.
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